AP2838b

antibody from Abcepta

Targeting: POLG2 HP55, MTPOLB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2838b

Provider

Abcepta

Proper citation

Abgent Cat#AP2838b, RRID:AB_10612704

Product name

POLG2 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of POLG2 Antibody (C-term) (Cat. #AP2838b) in HL-60 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

POLG2 Antibody (C-term) (Cat. #AP2838b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50