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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-37999 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAT8L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with human, rat, dog and cow based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAT8L by a NAT8L monoclonal antibody (Product # PA5-37999) at a concentration of 0.1 µg/mL. Brain of fetal Mouse (A) + peptide (B), Mouse (C) + peptide (D), Human (E) + peptide (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of NAT8L/N-acetylaspartate synthetase in fetal mouse brain lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a NAT8L/N-acetylaspartate synthetase polyclonal antibody (Product # PA5-37999) at a dilution of 0.1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NAT8L in HEK293 cells using a NAT8L monoclonal antibody (Product # PA5-37999) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NAT8L in Kelly cells using a NAT8L monoclonal antibody (Product # PA5-37999) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NAT8L in Kelly cells using a polyclonal antibody (Product #PA5-37999). Kelly cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
