Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5627 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse ZIP4H/Tex11 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ZIP4H/Tex11 in Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14AT2
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse ZIP4H/Tex11 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected, transfected with human ZIP4H, or transfected with mouse ZIP4H. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse ZIP4H/Tex11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5627) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZIP4H/Tex11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.