PAB27866

antibody from Abnova Corporation

Targeting: POGLUT3 KDELC2, MGC33424

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27866

Provider

Abnova Corporation

Product name

KDELC2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KDELC2.

Antigen sequence

YHEYCECPEDPQAWQKTLSCPTKEPQIAKDFASFP
SINLQQMLKEVPKRFGDERGAIVHYTILNNHVYRR
SLGKYTDFKMFSDEI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406972) with KDELC2 polyclonal antibody (Cat # PAB27866) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with KDELC2 polyclonal antibody (Cat # PAB27866) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli & vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with KDELC2 polyclonal antibody (Cat # PAB27866) shows strong cytoplasmic positivity in trophoblastic cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)