Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB8279 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat alpha-Actinin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha-Actinin 1 in ELISA, and human, mouse and rat alpha-Actinin 1 in Western Blot.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P12814
- Isotype
- IgG
- Antibody clone number
- 910301
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat alpha-Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha-Actinin 1 Monoclonal Antibody (Catalog # MAB8279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse alpha-Actinin 1 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.5 mg/mL. A specific band was detected for alpha-Actinin 1 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha-Actinin 1 Monoclonal Antibody (Catalog # MAB8279). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.