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Western blot
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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Other assay [1]
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- Product number
- 40-7400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Connexin 30.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- 40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.
Cardiomyocyte Expression of ZO-1 Is Essential for Normal Atrioventricular Conduction but Does Not Alter Ventricular Function.
Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition.
Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.
Cooler S, Schwartz GW
Nature neuroscience 2021 Jan;24(1):105-115
Nature neuroscience 2021 Jan;24(1):105-115
Cardiomyocyte Expression of ZO-1 Is Essential for Normal Atrioventricular Conduction but Does Not Alter Ventricular Function.
Zhang J, Vincent KP, Peter AK, Klos M, Cheng H, Huang SM, Towne JK, Ferng D, Gu Y, Dalton ND, Chan Y, Li R, Peterson KL, Chen J, McCulloch AD, Knowlton KU, Ross RS
Circulation research 2020 Jul 3;127(2):284-297
Circulation research 2020 Jul 3;127(2):284-297
Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition.
Bhattacharyya S, Duan J, Wang L, Li B, Bhakta M, Fernandez-Perez A, Hon GC, Munshi NV
Scientific reports 2019 Feb 14;9(1):2106
Scientific reports 2019 Feb 14;9(1):2106
Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.
Gemel J, Lin X, Collins R, Veenstra RD, Beyer EC
Biochemical and biophysical research communications 2008 May 2;369(2):388-94
Biochemical and biophysical research communications 2008 May 2;369(2):388-94
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Fig. 4 Immunohistochemistry for three types of Connexin at RGC contact points shows negative results Three connexins were evaluated for presence at the regions of contact between an F-mini-ON and multiple F-mini-OFF RGCs, n = 1 of each experiment. a,b, Full depth maximum intensity projection images of a Neurobiotin-filled F-mini-ON RGC (magenta),the connected F-mini-OFF RGCs (cyan), and a cell of unclassified type due to insufficiently filled dendrites (yellow). Tracing, segmentation, and masking were performed manually. Image brightness was scaled separately by cell type for illustration here but not for analysis. c,d Thin projection images of regions in orange squares in a,b showing an example RGC crossing point with yellow square for spatial reference. Stack depth is 3.5 mum. e-g, The same region and depth as in c,d, showing the IHC channels for the three connexin proteins. h, Quantification of overlap between connexin images and RGC contact region masks. Values are similar before and after a 90 degree rotation of the connexin image. Points mark the overlap of the single F-mini-ON RGC with each F-mini-OFF RGC in the image.
