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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX111584 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111584, RRID:AB_11164763
- Product name
- LYN antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells.
Activation of Lyn tyrosine kinase through decreased membrane cholesterol levels during a change in its membrane distribution upon cell detachment.
Morinaga T, Yanase S, Okamoto A, Yamaguchi N, Yamaguchi N
Scientific reports 2017 Mar 28;7(1):493
Scientific reports 2017 Mar 28;7(1):493
Activation of Lyn tyrosine kinase through decreased membrane cholesterol levels during a change in its membrane distribution upon cell detachment.
Morinaga T, Abe K, Nakayama Y, Yamaguchi N, Yamaguchi N
The Journal of biological chemistry 2014 Sep 19;289(38):26327-43
The Journal of biological chemistry 2014 Sep 19;289(38):26327-43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LYN antibody detects LYN protein by Western blot analysis.A. 30 ?g THP-1 whole cell lysate/extract7.5 % SDS-PAGELYN antibody (GTX111584) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX111584) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LYN antibody immunoprecipitates LYN protein in IP experiments.IP samples: K562 whole cell extractA. 40 £gg K562 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of LYN protein by 4 £gg LYN antibody (GTX111584)5 % SDS-PAGEThe immunoprecipitated LYN protein was detected by LYN antibody (GTX111584) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
