Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-49458 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXO45 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references DNAJB9 suppresses the metastasis of triple-negative breast cancer by promoting FBXO45-mediated degradation of ZEB1.
Kim HY, Kim YM, Hong S
Cell death & disease 2021 May 8;12(5):461
Cell death & disease 2021 May 8;12(5):461
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 DNAJB9 regulates ZEB1 through FBXO45-mediated ubiquitination. a To identify the target for ZEB1 degradation, shRNAs against SIAH1 or FBXO45 were introduced into MDA-MB-231 cells. Then the expression level of ZEB1 proteins was checked using western blotting. b The expression levels of FBXO45 proteins were checked using western blotting in MCF7-shDNAJB9 and MDA-MB-231-DNAJB9-OE cells. c The Pearson correlation between DNAJB9 and FBXO45 protein expression in 102 breast cancer tissues from The Cancer Genome Atlas database. The Pearson correlation coefficient ( R ) and p value ( p ) are reported. d To confirm the interaction between DNAJB9 and FBXO45, MCF7 and T47D cell lysates were subjected to immunoprecipitation with control IgG or anti-DNAJB9 antibodies. The immunoprecipitates were detected using the indicated antibodies. e To confirm the inhibitory effect of DNAJB9 on FBXO45 ubiquitination, MCF7-shDNAJB9 and MDA-MB-231-DNAJB9-OE cell lysates were subjected to immunoprecipitation with an anti-FBXO45 antibody. Then the immunoprecipitated FBXO45 was detected with an anti-ubiquitin antibody. f To validate the FBXO45-mediated ubiquitination of ZEB1, indicated cells were transfected using ZEB1 and ubiquitin constructs. Then the immunoprecipitated ZEB1 was detected with an anti-HA antibody. Results are presented as mean +- standard deviation from three independent experiments.
