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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3528 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3528, RRID:AB_1581834
- Product name
- VARS polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of VARS.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Three-dimensional reconstruction of the valyl-tRNA synthetase/elongation factor-1H complex and localization of the delta subunit.
Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Jiang S, Wolfe CL, Warrington JA, Norcum MT
FEBS letters 2005 Nov 7;579(27):6049-54
FEBS letters 2005 Nov 7;579(27):6049-54
Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Giegé R, Florentz C, Sissler M
Biochemistry 2005 Mar 29;44(12):4805-16
Biochemistry 2005 Mar 29;44(12):4805-16
Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Xie T, Rowen L, Aguado B, Ahearn ME, Madan A, Qin S, Campbell RD, Hood L
Genome research 2003 Dec;13(12):2621-36
Genome research 2003 Dec;13(12):2621-36
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of VARS polyclonal antibody (Cat # PAB3528) in K-562 cell line lysates (35 ug/lane).VARS (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with VARS polyclonal antibody (Cat # PAB3528), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
