Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30251 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (84%), Rat (86%), Pig (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VARS Polyclonal Antibody (Product # PA5-30251). Sample (30 µg of whole cell lysate). Lane A: Hep G2. Lane B: HCT116 . 5% SDS PAGE. VARS Polyclonal Antibody (Product # PA5-30251) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Valyl tRNA synthetase in methanol-fixed HeLa cells using a Valyl tRNA synthetase polyclonal antibody (Product # PA5-30251) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VARS Polyclonal Antibody detects Valyl tRNA synthetase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VARS Polyclonal Antibody (Product # PA5-30251) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.