PA5-59815

SHFL antibody from Invitrogen Antibodies

C19orf66, FLJ11286, IRAV, RyDEN, SFL

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for PA5-59815

Antibody Data

Product number

PA5-59815

Provider

Invitrogen Antibodies

Product name

C19orf66 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting.
Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU
Proceedings of the National Academy of Sciences of the United States of America 2021 Jun 29;118(26)

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Restriction of SARS-CoV-2 Replication by Targeting Programmed -1 Ribosomal Frameshifting In Vitro.
Sun Y, Abriola L, Surovtseva YV, Lindenbach BD, Guo JU
bioRxiv : the preprint server for biology 2020 Oct 21;

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C19orf66 in human cell lines SK-MEL-30 and HEK293 using a C19orf66 Polyclonal Antibody (Product # PA5-59815). Corresponding C19orf66 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C19orf66 in human cell lines SK-MEL-30 and HEK293 using a C19orf66 Polyclonal Antibody (Product # PA5-59815). Corresponding C19orf66 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C19orf66 in human cell lines SK-MEL-30 and HEK293 using a C19orf66 Polyclonal Antibody (Product # PA5-59815). Corresponding C19orf66 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of C19orf66 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a C19orf66 Polyclonal Antibody (Product # PA5-59815).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of C19orf66 in human cell line U-2 OS using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows localization to nucleoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of C19orf66 in human cell line U-2 OS using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows localization to nucleoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C19orf66 in human liver using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows strong cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C19orf66 in human fallopian tube using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C19orf66 in human prostate using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C19orf66 in human testis using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows moderate cytoplasmic positivity in Leydig cells and very weak cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C19orf66 in human testis using C19orf66 Polyclonal Antibody (Product # PA5-59815) shows moderate cytoplasmic positivity in Leydig cells and very weak cytoplasmic positivity in cells in seminiferous ducts.