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- Product number
- PA5-72040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DSPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells.
Li J, Deng Q, Fan W, Zeng Q, He H, Huang F
Bioengineered 2020 Dec;11(1):829-840
Bioengineered 2020 Dec;11(1):829-840
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of (overlay histogram) of DSPP in PC-3 cells (green line). Samples were incubated with DSPP polyclonal antibody (Product # PA5-72040) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at Room temperature. The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. The effect of melatonin on the expression of odontogenic markers and DNMTs during odontogenic differentiation of hDPCs. The mRNA expression of odontogenic markers, (a) DSPP , (b) DMP-1 , and DNMTs, (e) DNMT1 , (f) DNMT3B , (g) DNMT3A was assessed by Real-time qPCR on day 7.The protein expression of odontogenic markers, (c) DSPP, (d) DMP-1, and DNMTs, (h) DNMT1, (i) DNMT3B was analyzed by western blotting on day 7. beta-actin was used as an internal control. The band intensities were analyzed using ImageJ software. All results are presented as the mean +- SEM (standard error of mean) from at least three independent experiments. Procedures were performed as described in the text. * P < 0.05 indicates a statistically significant difference.
