Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 22503-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#22503-1-AP, RRID:AB_11232413
- Product name
- CNOT9 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CNOT9 antibody (Cat. #22503-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references High-throughput tagging of endogenous loci for rapid characterization of protein function.
Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4-NOT.
Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.
Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability.
Post-translational control of beige fat biogenesis by PRDM16 stabilization.
The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway.
The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.
TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.
Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.
RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.
Phase Transitions in the Assembly and Function of Human miRISC.
Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.
Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.
Kim J, Kratz AF, Chen S, Sheng J, Kim HK, Zhang L, Singh BK, Chavez A
Science advances 2024 May 3;10(18):eadg8771
Science advances 2024 May 3;10(18):eadg8771
Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4-NOT.
Absmeier E, Chandrasekaran V, O'Reilly FJ, Stowell JAW, Rappsilber J, Passmore LA
Nature structural & molecular biology 2023 Sep;30(9):1314-1322
Nature structural & molecular biology 2023 Sep;30(9):1314-1322
Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.
Höpfler M, Absmeier E, Peak-Chew SY, Vartholomaiou E, Passmore LA, Gasic I, Hegde RS
Molecular cell 2023 Jul 6;83(13):2290-2302.e13
Molecular cell 2023 Jul 6;83(13):2290-2302.e13
Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability.
Hagkarim NC, Hajkarim MC, Suzuki T, Fujiwara T, Winkler GS, Stewart GS, Grand RJ
Cells 2023 Jul 17;12(14)
Cells 2023 Jul 17;12(14)
Post-translational control of beige fat biogenesis by PRDM16 stabilization.
Wang Q, Li H, Tajima K, Verkerke ARP, Taxin ZH, Hou Z, Cole JB, Li F, Wong J, Abe I, Pradhan RN, Yamamuro T, Yoneshiro T, Hirschhorn JN, Kajimura S
Nature 2022 Sep;609(7925):151-158
Nature 2022 Sep;609(7925):151-158
The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway.
Carreño A, Lykke-Andersen J
Molecular and cellular biology 2022 Sep 15;42(9):e0005522
Molecular and cellular biology 2022 Sep 15;42(9):e0005522
The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.
Zou L, Moch C, Graille M, Chapat C
iScience 2022 Jul 15;25(7):104646
iScience 2022 Jul 15;25(7):104646
TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.
Matkovic R, Morel M, Lanciano S, Larrous P, Martin B, Bejjani F, Vauthier V, Hansen MMK, Emiliani S, Cristofari G, Gallois-Montbrun S, Margottin-Goguet F
Nature communications 2022 Jan 10;13(1):66
Nature communications 2022 Jan 10;13(1):66
Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.
Munakata F, Suzawa M, Ui-Tei K
Genes 2021 Feb 13;12(2)
Genes 2021 Feb 13;12(2)
RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.
Poetz F, Corbo J, Levdansky Y, Spiegelhalter A, Lindner D, Magg V, Lebedeva S, Schweiggert J, Schott J, Valkov E, Stoecklin G
Nature communications 2021 Dec 9;12(1):7175
Nature communications 2021 Dec 9;12(1):7175
Phase Transitions in the Assembly and Function of Human miRISC.
Sheu-Gruttadauria J, MacRae IJ
Cell 2018 May 3;173(4):946-957.e16
Cell 2018 May 3;173(4):946-957.e16
Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim SH, Attig J, Shapiro M, Morita M, Khoutorsky A, Alain T, Gkogkas CG, Stern-Ginossar N, Tuschl T, Gingras AC, Duchaine TF, Sonenberg N
eLife 2018 Feb 7;7
eLife 2018 Feb 7;7
Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.
Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
Nucleic acids research 2017 Jan 25;45(2):938-950
Nucleic acids research 2017 Jan 25;45(2):938-950
Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Hicks JA, Li L, Matsui M, Chu Y, Volkov O, Johnson KC, Corey DR
Cell reports 2017 Aug 15;20(7):1543-1552
Cell reports 2017 Aug 15;20(7):1543-1552
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
Mathys H, Basquin J, Ozgur S, Czarnocki-Cieciura M, Bonneau F, Aartse A, Dziembowski A, Nowotny M, Conti E, Filipowicz W
Molecular cell 2014 Jun 5;54(5):751-65
Molecular cell 2014 Jun 5;54(5):751-65
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.
Chen Y, Boland A, Kuzuoğlu-Öztürk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E
Molecular cell 2014 Jun 5;54(5):737-50
Molecular cell 2014 Jun 5;54(5):737-50
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22503-1-AP(RQCD1 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RQCD1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human RQCD1. This antibody recognizes human,mouse,rat antigen. The RQCD1 antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.
