HPA023370

antibody from Atlas Antibodies

Targeting: PCM1 PTC4

Provider product page for HPA023370

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [42]
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Validations
Immunocytochemistry [1]

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Product number: HPA023370 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA023370, RRID:AB_1855072
Product name: Anti-PCM1
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human PCM1, Gene description: pericentriolar material 1, Alternative Gene Names: PTC4, Validated applications: IHC, ICC, Uniprot ID: Q15154, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

PCM1 protein structure - HPA023370 shown in red.

Submitted references Cross-species comparison reveals that Hmga1 reduces H3K27me3 levels to promote cardiomyocyte proliferation and cardiac regeneration.

Bouwman M, de Bakker DEM, Honkoop H, Giovou AE, Versteeg D, Boender AR, Nguyen PD, Slotboom M, Colquhoun D, Vigil-Garcia M, Kooijman L, Janssen R, Hooijkaas IB, Günthel M, Visser KJ, Klerk M, Zentilin L, Giacca M, Kaslin J, Boink GJJ, van Rooij E, Christoffels VM, Bakkers J
Nature cardiovascular research 2025 Jan;4(1):64-82

Deep Learning Resolves Myovascular Dynamics in the Failing Human Heart

Karpurapu A, Williams H, DeBenedittis P, Baker C, Ren S, Thomas M, Beard A, Devlin G, Harrington J, Parker L, Smith A, Mainsah B, Pla M, Asokan A, Bowles D, Iversen E, Collins L, Karra R
JACC: Basic to Translational Science 2024;9(5):674-686

Masuzawa R, Rosa Flete H, Shimizu J, Kawano F
The Journal of Physiological Sciences 2024;74(1):41

A broadly applicable method for quantifying cardiomyocyte cell division identifies proliferative events following myocardial infarction

Swift S, Purdy A, Buddell T, Lovett J, Chanjeevaram S, Arkatkar A, O’Meara C, Patterson M
Cell Reports Methods 2024;4(9):100860

Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics

Hashimoto K, Ohira M, Kodama A, Kimoto M, Inoue M, Toné S, Usui Y, Hanashima A, Goto T, Ogura Y, Ujihara Y, Mohri S
Scientific Reports 2024;14(1)

En A, Bogireddi H, Thomas B, Stutzman A, Ikegami S, LaForest B, Almakki O, Pytel P, Moskowitz I, Ikegami K
2024

A Prochlorperazine-Induced Decrease in Autonomous Muscle Activity during Hindlimb Unloading Is Accompanied by Preserved Slow Myosin mRNA Expression

Sharlo K, Lvova I, Tyganov S, Sergeeva K, Kalashnikov V, Kalashnikova E, Mirzoev T, Kalamkarov G, Shevchenko T, Shenkman B
Current Issues in Molecular Biology 2023;45(7):5613-5630

Development of new adeno-associated virus capsid variants for targeted gene delivery to human cardiomyocytes

Kok C, Tsurusaki S, Cabanes-Creus M, Igoor S, Rao R, Skelton R, Liao S, Ginn S, Knight M, Scott S, Mietzsch M, Fitzsimmons R, Miller J, Mohamed T, McKenna R, Chong J, Hill A, Hudson J, Alexander I, Lisowski L, Kizana E
Molecular Therapy - Methods & Clinical Development 2023;30

AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

Kneppers A, Ben Larbi S, Theret M, Saugues A, Dabadie C, Gsaier L, Ferry A, Rhein P, Gondin J, Sakamoto K, Mounier R
iScience 2023;26(12):108343

An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility

Bosada F, van Duijvenboden K, Giovou A, Rivaud M, Uhm J, Verkerk A, Boukens B, Christoffels V
eLife 2023;12

Genetic inactivation of β-catenin is salubrious, whereas its activation is deleterious in desmoplakin cardiomyopathy

Olcum M, Fan S, Rouhi L, Cheedipudi S, Cathcart B, Jeong H, Zhao Z, Gurha P, Marian A
Cardiovascular Research 2023;119(17):2712-2728

HRas and Myc synergistically induce cell cycle progression and apoptosis of murine cardiomyocytes.

Boikova A, Bywater MJ, Quaife-Ryan GA, Straube J, Thompson L, Ascanelli C, Littlewood TD, Evan GI, Hudson JE, Wilson CH
Frontiers in cardiovascular medicine 2022;9:948281

Acute Glycogen Synthase Kinase-3 Inhibition Modulates Human Cardiac Conduction

Li G, Brumback B, Huang L, Zhang D, Yin T, Lipovsky C, Hicks S, Jimenez J, Boyle P, Rentschler S
JACC: Basic to Translational Science 2022;7(10):1001-1017

Depressed Protein Synthesis and Anabolic Signaling Potentiate ACL Tear–Resultant Quadriceps Atrophy

Keeble A, Brightwell C, Latham C, Thomas N, Mobley C, Murach K, Johnson D, Noehren B, Fry C
The American Journal of Sports Medicine 2022;51(1):81-96

Postnatal expression of cell cycle promoter Fam64a causes heart dysfunction by inhibiting cardiomyocyte differentiation through repression of Klf15

Hashimoto K, Kodama A, Ohira M, Kimoto M, Nakagawa R, Usui Y, Ujihara Y, Hanashima A, Mohri S
iScience 2022;25(5):104337

Coupled myovascular expansion directs cardiac growth and regeneration

DeBenedittis P, Karpurapu A, Henry A, Thomas M, McCord T, Brezitski K, Prasad A, Baker C, Kobayashi Y, Shah S, Kontos C, Tata P, Lumbers R, Karra R
Development 2022;149(18)

Isolation and Characterization of Primary DMD Pig Muscle Cells as an In Vitro Model for Preclinical Research on Duchenne Muscular Dystrophy

Donandt T, Hintze S, Krause S, Wolf E, Schoser B, Walter M, Meinke P
Life 2022;12(10):1668

Inhibition of β1-AR/Gαs signaling promotes cardiomyocyte proliferation in juvenile mice through activation of RhoA-YAP axis

Sakabe M, Thompson M, Chen N, Verba M, Hassan A, Lu R, Xin M
eLife 2022;11

The WNT/β-catenin pathway regulates expression of the genes involved in cell cycle progression and mitochondrial oxidative phosphorylation in the postmitotic cardiac myocytes

Olcum M, Cheedipudi S, Rouhi L, Fan S, Jeong H, Zhao Z, Gurha P, Marian A
The Journal of Cardiovascular Aging 2022

Comparing the epigenetic landscape in myonuclei purified with a PCM1 antibody from a fast/glycolytic and a slow/oxidative muscle

Blewitt M, Bengtsen M, Winje I, Eftestøl E, Landskron J, Sun C, Nygård K, Domanska D, Millay D, Meza-Zepeda L, Gundersen K
PLOS Genetics 2021;17(11):e1009907

A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration

Raso A, Dirkx E, Sampaio-Pinto V, el Azzouzi H, Cubero R, Sorensen D, Ottaviani L, Olieslagers S, Huibers M, de Weger R, Siddiqi S, Moimas S, Torrini C, Zentillin L, Braga L, Nascimento D, da Costa Martins P, van Berlo J, Zacchigna S, Giacca M, De Windt L
Nature Communications 2021;12(1)

Replication Stress Response Modifies Sarcomeric Cardiomyopathy Remodeling

Pal S, Nixon B, Glennon M, Shridhar P, Satterfield S, Su Y, Becker J
Journal of the American Heart Association 2021;10(15)

Epigenetic State Changes Underlie Metabolic Switch in Mouse Post-Infarction Border Zone Cardiomyocytes.

Günthel M, van Duijvenboden K, de Bakker DEM, Hooijkaas IB, Bakkers J, Barnett P, Christoffels VM
Journal of cardiovascular development and disease 2021 Oct 22;8(11)

Early Postnatal Cardiac Stress Does Not Influence Ventricular Cardiomyocyte Cell-Cycle Withdrawal.

Günthel M, van Duijvenboden K, Jeremiasse J, van den Hoff MJB, Christoffels VM
Journal of cardiovascular development and disease 2021 Apr 7;8(4)

Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes

Yucel N, Axsom J, Yang Y, Li L, Rhoades J, Arany Z
eLife 2020;9

A calcineurin–Hoxb13 axis regulates growth mode of mammalian cardiomyocytes

Nguyen N, Canseco D, Xiao F, Nakada Y, Li S, Lam N, Muralidhar S, Savla J, Hill J, Le V, Zidan K, El-Feky H, Wang Z, Ahmed M, Hubbi M, Menendez-Montes I, Moon J, Ali S, Le V, Villalobos E, Mohamed M, Elhelaly W, Thet S, Anene-Nzelu C, Tan W, Foo R, Meng X, Kanchwala M, Xing C, Roy J, Cyert M, Rothermel B, Sadek H
Nature 2020;582(7811):271-276

Chamber-specific transcriptional responses in atrial fibrillation

Lipovsky C, Jimenez J, Guo Q, Li G, Yin T, Hicks S, Bhatnagar S, Takahashi K, Zhang D, Brumback B, Goldsztejn U, Nadadur R, Perez-Cervantez C, Moskowitz I, Liu S, Zhang B, Rentschler S
JCI Insight 2020;5(18)

LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration

Wu Y, Zhou L, Liu H, Duan R, Zhou H, Zhang F, He X, Lu D, Xiong K, Xiong M, Zhuang J, Liu Y, Li L, Liang D, Chen Y
Cell Research 2020;31(4):450-462

Reactivation of Myc transcription in the mouse heart unlocks its proliferative capacity

Bywater M, Burkhart D, Straube J, Sabò A, Pendino V, Hudson J, Quaife-Ryan G, Porrello E, Rae J, Parton R, Kress T, Amati B, Littlewood T, Evan G, Wilson C
Nature Communications 2020;11(1)

PCM1 is necessary for focal ciliary integrity and is a candidate for severe schizophrenia

Monroe T, Garrett M, Kousi M, Rodriguiz R, Moon S, Bai Y, Brodar S, Soldano K, Savage J, Hansen T, Muzny D, Gibbs R, Barak L, Sullivan P, Ashley-Koch A, Sawa A, Wetsel W, Werge T, Katsanis N
Nature Communications 2020;11(1)

Exercise promotes satellite cell contribution to myofibers in a load-dependent manner

Masschelein E, D’Hulst G, Zvick J, Hinte L, Soro-Arnaiz I, Gorski T, von Meyenn F, Bar-Nur O, De Bock K
Skeletal Muscle 2020;10(1)

YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo

Monroe T, Hill M, Morikawa Y, Leach J, Heallen T, Cao S, Krijger P, de Laat W, Wehrens X, Rodney G, Martin J
Developmental Cell 2019;48(6):765-779.e7

PAN-INTACT enables direct isolation of lineage-specific nuclei from fibrous tissues.

Bhattacharyya S, Sathe AA, Bhakta M, Xing C, Munshi NV
PloS one 2019;14(4):e0214677

Genomic Reorganization of Lamin-Associated Domains in Cardiac Myocytes Is Associated With Differential Gene Expression and DNA Methylation in Human Dilated Cardiomyopathy

Cheedipudi S, Matkovich S, Coarfa C, Hu X, Robertson M, Sweet M, Taylor M, Mestroni L, Cleveland J, Willerson J, Gurha P, Marian A
Circulation Research 2019;124(8):1198-1213

Identification of atrial fibrillation associated genes and functional non-coding variants

van Ouwerkerk A, Bosada F, van Duijvenboden K, Hill M, Montefiori L, Scholman K, Liu J, de Vries A, Boukens B, Ellinor P, Goumans M, Efimov I, Nobrega M, Barnett P, Martin J, Christoffels V
Nature Communications 2019;10(1)

Distinct epigenetic programs regulate cardiac myocyte development and disease in the human heart in vivo

Gilsbach R, Schwaderer M, Preissl S, Grüning B, Kranzhöfer D, Schneider P, Nührenberg T, Mulero-Navarro S, Weichenhan D, Braun C, Dreßen M, Jacobs A, Lahm H, Doenst T, Backofen R, Krane M, Gelb B, Hein L
Nature Communications 2018;9(1)

Hippo pathway deficiency reverses systolic heart failure after infarction

Leach J, Heallen T, Zhang M, Rahmani M, Morikawa Y, Hill M, Segura A, Willerson J, Martin J
Nature 2017;550(7675):260-264

Alterations in sarcomere function modify the hyperplastic to hypertrophic transition phase of mammalian cardiomyocyte development

Nixon B, Williams A, Glennon M, de Feria A, Sebag S, Baldwin H, Becker J
JCI Insight 2017;2(4)

Epicardial FSTL1 reconstitution regenerates the adult mammalian heart

Wei K, Serpooshan V, Hurtado C, Diez-Cuñado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P
Nature 2015;525(7570):479-485

Cardiac Myocyte De Novo DNA Methyltransferases 3a/3b Are Dispensable for Cardiac Function and Remodeling after Chronic Pressure Overload in Mice

Hirsch E, Nührenberg T, Hammann N, Schnick T, Preißl S, Witten A, Stoll M, Gilsbach R, Neumann F, Hein L
PLOS ONE 2015;10(6):e0131019

Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease

Gilsbach R, Preissl S, Grüning B, Schnick T, Burger L, Benes V, Würch A, Bönisch U, Günther S, Backofen R, Fleischmann B, Schübeler D, Hein L
Nature Communications 2014;5(1)

RBM 14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity

Shiratsuchi G, Takaoka K, Ashikawa T, Hamada H, Kitagawa D
The EMBO Journal 2014;34(1):97-114

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line A-431 shows localization to centrosome.
Sample type: Human