NBP1-87195

antibody from Novus Biologicals

Targeting: PCM1 PTC4

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87195

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87195, RRID:AB_11035743

Product name

Rabbit Polyclonal PCM1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human PCM1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Cardiac Myocyte De Novo DNA Methyltransferases 3a/3b Are Dispensable for Cardiac Function and Remodeling after Chronic Pressure Overload in Mice.

Nührenberg TG, Hammann N, Schnick T, Preißl S, Witten A, Stoll M, Gilsbach R, Neumann FJ, Hein L
PloS one 2015;10(6):e0131019

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Epicardial FSTL1 reconstitution regenerates the adult mammalian heart.

Wei K, Serpooshan V, Hurtado C, Diez-Cuñado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider MD, van den Hoff MJ, Butte MJ, Yang PC, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P
Nature 2015 Sep 24;525(7570):479-85

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RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity.

Shiratsuchi G, Takaoka K, Ashikawa T, Hamada H, Kitagawa D
The EMBO journal 2015 Jan 2;34(1):97-114

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Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease.

Gilsbach R, Preissl S, Grüning BA, Schnick T, Burger L, Benes V, Würch A, Bönisch U, Günther S, Backofen R, Fleischmann BK, Schübeler D, Hein L
Nature communications 2014 Oct 22;5:5288

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human testis shows moderate cytoplasmic positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human ovary.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87195 (A) shows similar protein distribution across tissues to independent antibody NBP1-87196 (B).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PCM1 Antibody [NBP1-87195] - Cardiomyocyte-specific Cre-mediated recombination. Representative flow cytometry plots depicting the gating strategy for sorting of cardiomyocyte nuclei. Positive 7-AAD (left panel) and PCM-1 (middle panel) staining identified cardiomyocyte nuclei. Purity of sorted cardiomyocyte nuclei is demonstrated in the right panel. FSC represents forward scatter. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0131019), licensed under a CC-BY licence.