F54931

antibody from NSJ Bioreagents

Targeting: PCM1 PTC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54931

Provider

NSJ Bioreagents

Product name

Pericentriolar material 1 protein Antibody / PCM1

Antibody type

Polyclonal

Description

This highly specific Pericentriolar material 1 protein antibody is suitable for use in Immunofluorescence/Flow cytometry/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Pericentriolar material 1 protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Ramos cell lysate with Pericentriolar material 1 protein antibody. Predicted molecular weight ~229 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human A549 cells with Pericentriolar material 1 protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast carcinoma tissue with Pericentriolar material 1 protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Ramos cells with Pericentriolar material 1 protein antibody; Blue=isotype control, Green= Pericentriolar material 1 protein antibody.