Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-58828 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58828, RRID:AB_2160198
- Product name
- Rabbit Polyclonal PCM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A synthetic peptide corresponding to a region of the human pericentriolar material 1 (PCM-1) protein binds β-amyloid (Aβ1-42 ) oligomers.
Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.
Gamma-tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells.
Chakravarthy B, Ménard M, Brown L, Hewitt M, Atkinson T, Whitfield J
Journal of neurochemistry 2013 Aug;126(3):415-24
Journal of neurochemistry 2013 Aug;126(3):415-24
Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.
Moynihan KL, Pooley R, Miller PM, Kaverina I, Bader DM
Molecular biology of the cell 2009 Nov;20(22):4790-803
Molecular biology of the cell 2009 Nov;20(22):4790-803
Gamma-tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells.
Kuriyama R, Bettencourt-Dias M, Hoffmann I, Arnold M, Sandvig L
Journal of cell science 2009 Jun 15;122(Pt 12):2014-23
Journal of cell science 2009 Jun 15;122(Pt 12):2014-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCM1 Antibody [NB100-58828] - Detection of Human and Mouse PCM1 on HeLa whole cell lysate using NB100-58828. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58829.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody NB100-58828 (lot NB100-58828-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-58828-1) and rabbit anti-PCM1 antibody NB100-58829. For blotting immunoprecipitated PCM1, NB100-58828 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.