19856-1-AP

antibody from Proteintech Group

Targeting: PCM1 PTC4

Provider product page for 19856-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [19]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]

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Product number: 19856-1-AP - Provider product page
Provider: Proteintech Group
Product name: PCM1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PCM1 antibody (Cat. #19856-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PCM1 protein structure - 19856-1-AP shown in red.

Submitted references A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.

Turan FB, Ercan ME, Firat-Karalar EN
Methods in molecular biology (Clifton, N.J.) 2024;2725:55-78

Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy.

Tang Y, Feng M, Su Y, Ma T, Zhang H, Wu H, Wang X, Shi S, Zhang Y, Xu Y, Hu S, Wei K, Xu D
Circulation 2023 May 30;147(22):1684-1704

Actin filaments form a size-dependent diffusion barrier around centrosomes.

Cheng H, Kao YL, Chen T, Sharma L, Yang WT, Chuang YC, Huang SH, Lin HR, Huang YS, Kao CL, Yang LW, Bearon R, Cheng HC, Hsia KC, Lin YC
EMBO reports 2023 Jan 9;24(1):e54935

TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins.

Nguyen A, Goetz SC
Molecular biology of the cell 2023 Jan 1;34(1):ar8

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.

Hall EA, Kumar D, Prosser SL, Yeyati PL, Herranz-Pérez V, García-Verdugo JM, Rose L, McKie L, Dodd DO, Tennant PA, Megaw R, Murphy LC, Ferreira MF, Grimes G, Williams L, Quidwai T, Pelletier L, Reiter JF, Mill P
eLife 2023 Feb 15;12

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Kumar D, Rains A, Herranz-Pérez V, Lu Q, Shi X, Swaney DL, Stevenson E, Krogan NJ, Huang B, Westlake C, Garcia-Verdugo JM, Yoder BK, Reiter JF
The Journal of cell biology 2021 Sep 6;220(9)

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Quidwai T, Wang J, Hall EA, Petriman NA, Leng W, Kiesel P, Wells JN, Murphy LC, Keighren MA, Marsh JA, Lorentzen E, Pigino G, Mill P
eLife 2021 Nov 4;10

Novel loss-of-function mutation in HERC2 is associated with severe developmental delay and paediatric lethality.

Elpidorou M, Best S, Poulter JA, Hartill V, Hobson E, Sheridan E, Johnson CA
Journal of medical genetics 2021 May;58(5):334-341

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Cohen CD, De Blasio MJ, Lee MKS, Farrugia GE, Prakoso D, Krstevski C, Deo M, Donner DG, Kiriazis H, Flynn MC, Gaynor TL, Murphy AJ, Drummond GR, Pinto AR, Ritchie RH
Cardiovascular diabetology 2021 Jun 1;20(1):116

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Arslanhan MD, Rauniyar N, Yates JR 3rd, Firat-Karalar EN
EMBO reports 2021 Aug 4;22(8):e51902

Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.

Zhang C, Li C, Siu GKY, Luo X, Yu S
Frontiers in cell and developmental biology 2020;8:148

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Gurkaslar HK, Culfa E, Arslanhan MD, Lince-Faria M, Firat-Karalar EN
Cell reports 2020 May 12;31(6):107630

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Conkar D, Bayraktar H, Firat-Karalar EN
Scientific reports 2019 Oct 3;9(1):14250

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Odabasi E, Gul S, Kavakli IH, Firat-Karalar EN
EMBO reports 2019 Jun;20(6)

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L
Nature communications 2017 Mar 31;8:14866

The X-linked deubiquitinase USP9X is an integral component of centrosome.

Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, Zhou Y, Ge W
The Journal of biological chemistry 2017 Aug 4;292(31):12874-12884

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Conkar D, Culfa E, Odabasi E, Rauniyar N, Yates JR 3rd, Firat-Karalar EN
Journal of cell science 2017 Apr 15;130(8):1450-1462

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 19856-1-AP(PCM1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP result of anti-PCM1 (19856-1-AP for IP and Detection) with HeLa cells.
Sample type: cell line