PA5-65609

antibody from Invitrogen Antibodies

Targeting: SPINDOC C11orf84, SPIN-DOC

Provider product page for PA5-65609

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

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Product number: PA5-65609 - Provider product page
Provider: Invitrogen Antibodies
Product name: C11orf84 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: PGNKKPRGQRW KEPPGEEPVR KKRGRPMTKN LDPDPEPPSP DSPTETFAAP AEVRHFTDGS FPAGFVLQLF SHTQLRGPDS KDSPKDREVA EG Highest antigen sequence identity to the following orthologs - mouse 81%, rat 80%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.05 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SPINDOC protein structure - PA5-65609 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Spindoc is predominantly expressed in testis. (A) Violin plots showing the mRNA expression pattern of Spindoc in multiple human organs in the GTEx database (TPM, Transcripts Per Million). (B) RT-qPCR analyses of Spindoc mRNA levels across ten organs from adult wild-type (WT) mice. Data are presented as mean +- SEM, n = 3. ( C ) Western blot showing the expression levels of Spindoc protein among ten organs of WT adult mice. GAPDH served as a loading control. ( D ) Densitometric quantification of Spindoc protein levels as in ( C ). Data are presented as mean +- SEM, n = 3. ( E ) RT-qPCR analyses of Spindoc mRNA levels in developing testes. Testes at postnatal Day 3 (P3), P5, P7, P10, P12, P14, P17, P21, P35, P42 and P56 were analyzed. Data are presented as mean +- SEM, n = 3. ( F ) Western blot illustrating the Spindoc protein levels in developing testes. Testes at postnatal Day 3 (P3), P5, P7, P10, P12, P14, P17, P21, P35, P42 and P56 were analyzed. GAPDH served as a loading control. ( G ) Dynamic expression pattern of Spindoc mRNA from single cell RNA-seq analyses in adult human testes []. SSC, Spermatogonial Stem cells. ( H ) Dynamic expression levels of Spindoc mRNA from single cell RNA-seq analyses in RA-synchronized testicular cells []. A1, type A1 spermatogonia; In, intermediate spermatogonia; BS, S phase type B spermatogonia; ePL, early preleptotene; mPL, middle preleptotene; lPL, Late preleptotene; L, leptotene; Z, zygotene; eP, early pachytene; mP, middle pachy

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Generation of Spindoc knockout mouse models. ( A ) Schematic diagram shows the targeting strategy for generating Spindoc knockout (KO) mice using the CRISPR/Cas9 system. Blue boxes represent exons of the Spindoc gene on mouse chromosome 19. The genomic sequence targeted by a pair of sgRNAs are underlined with PAM sequences being highlighted in purple color. The red arrowheads point to the cut sites around exon 2 of Spindoc gene. Two F0 founder lines were generated - Line 1 carried 1 nt (T) insertion, while Line 2 carried a combination of 22 bp deletions as indicated; ( B, C ) Sanger sequencing of genomic DNA from mouse tail clips showing the frameshift variant (+ 1 nt) of KO mice (Line 1) (B), and the other frameshift variant (- 22 nt) (Line 2) (C); ( D ) Representative western blot analyses validated the Spindoc protein levels in WT and KO adult testes. GAPDH was used as a loading control. ( E ) Quantitative analysis of Spindoc protein levels in WT and KO adult testes. Data are presented as mean +- SEM, n = 3. P < 0.001 by student t-test . ( F ) Representative morphology of testes and epididymis from WT, Heterozygote (HET) and KO mice. The testis of KO was smaller than that of the WT; the epididymis of KO was more transparent than that of the WT. ( G ) Histogram showing the testis weights in WT and KO adult mice. Data are presented as mean +- SEM, n = 3. p < 0.001 by student t-test