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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MA5-24313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEU1 Monoclonal Antibody (688215)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 688215
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human NEU-1/Sialidase-1 Monoclonal Antibody (Product # MA5-24313) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEU1 in Jurkat human acute T cell leukemia cell line and human pancreas tissue. Samples were incubated in NEU1 monoclonal antibody (Product # MA5-24313) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for NEU‚1/Sialidase‚1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions.
