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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-55882 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BANK1 Monoclonal Antibody (K92023_11B1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: PGTMVEGQME RSQNWGHPGV RQETGDEPKG EKEKKEEEKE QEEEEDPYTF AEIDDSEYDM ILANLSIKKK TGSRSFIINR PPAPTPRPTS IPPKEETTPY IAQVFQQKTA RRQSDDDKFC GLPKKQDRAR IESPAFSTLR GCLTDGQEEL ILLQEKVKNG
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K92023_11B1
- Vial size
- 50 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of BANK1 in paraffin-embedded tonsil tissue. Sample was incubated with BANK1 monoclonal antibody (Product # MA5-55882) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
