Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-25141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Xenopus based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPC2 in U-2OS whole cell lysate. Samples were incubated with EPC2 polyclonal antibody (Product # PA5-25141) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 91 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPC2 in mouse cerebellum tissue lysates. Samples were incubated with EPC2 polyclonal antibody (Product # PA5-25141). Lysates: 35 µg/lane. EPC2 (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of 293 cells using an EPC2 polyclonal antibody (Product # PA5-25141) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of EPC2 in 293 cells (right histogram). Samples were incubated with EPC2 polyclonal antibody (Product # PA5-25141) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
