TA335793

antibody from OriGene

Targeting: SMARCA1 hSNF2L, ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SWI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA335793

Provider

OriGene

Product name

Rabbit Polyclonal Anti-SMARCA1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-SMARCA1 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SMARCA1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB Suggested Anti-SMARCA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate. SMARCA1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Rabbit Anti-SMARCA1 antibody; Paraffin Embedded Tissue: Human Heart cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X

Validation comment

IHC

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) Using SMARCA1 antibody - N-terminal region and HCT116 Cells

Validation comment

Assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Validation comment

Assay