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antibody from Invitrogen Antibodies
Targeting: SMARCA1
hSNF2L, ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SWI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- PA5-41440 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The transcription factor Zfp90 regulates the self-renewal and differentiation of hematopoietic stem cells.
Liu T, Kong WX, Tang XY, Xu M, Wang QH, Zhang B, Hu LD, Chen H
Cell death & disease 2018 Jun 7;9(6):677
Cell death & disease 2018 Jun 7;9(6):677
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HEK293T cell lysate using an anti-SMARCA1 polyclonal antibody (Product # PA5-41440).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human heart tissue using an anti-SMARCA1 polyclonal antibody (Product # PA5-41440).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay analysis of HCT116 cells using an anti-SMARCA1 polyclonal antibody (Product # PA5-41440).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay analysis of quiescent human colon carcinoma HCT116 cells using an anti-SMARCA1 polyclonal antibody (Product # PA5-41440).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Zfp90 cooperates with the NURF complex to regulate Hoxa9 expression. a Relative expression of representative HSC-proliferation-related genes. HSCs were isolated from Zfp90 +/+ and Zfp90 -/- mice 16 weeks after transplantation. b - d Analysis of representative gene expression in Bptf -, Snf2l - or Rbbp4 -deleted HSCs and WT control. Two sgRNAs were used for the deletion of Bptf, Snf2l or Rbbp4. e Analysis of Zfp90 enrichment on Hoxa9 promoter in LSK (Lin - Sca-1 + c-Kit + ) cells via ChIP assays. f Analysis of the direct interaction of Zfp90 with Hoxa9 promoter via EMSA assays. g Myc-Zfp90, pTK, and pGL3- Hoxa9 WT (region: -2000 to 0) or Mutant (Mut region: deletion of -550 to -800) promoter were transfected into 293T cells for luciferase assay. h , i Analysis of Bptf or Snf2l enrichment on Hoxa9 promoter in Zfp90 +/+ or Zfp90 -/- LSKs. j Accessibility of Hoxa9 promoter to DNase I in Zfp90 +/+ or Zfp90 -/- LSKs was assessed. * p
