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LearnMA5-31912
antibody from Invitrogen Antibodies
Targeting: SMARCA1
hSNF2L, ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SWI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-31912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCA1 Monoclonal Antibody (2H7B9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31912 has been tested in indirect ELISA.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H7B9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA1 in PANC-1 (1), HEK293 (2), SW620 (3), HT-29 (4), SH-SY5Y (5), and SK-OV-3 (6) cell lysate. Samples were incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA1 in human SMARCA1 recombinant protein. Sample was incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA1 in HEK293 (1) and SMARCA1 cell lysate. Samples were incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of SMARCA1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SMARCA1 in paraffin-embedded esophageal cancer tissue. Sample was incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SMARCA1 in paraffin-embedded stomach cancer tissue. Sample was incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of SMARCA1 in NIH/3T3 cells (green). Sample was incubated with SMARCA1 monoclonal antibody (Product # MA5-31912) using a dilution of 1:200-1:400 followed by negative control (red).
