NB100-57524

antibody from Novus Biologicals

Targeting: SMARCA1 hSNF2L, ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SWI

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-57524

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-57524, RRID:AB_2302393

Product name

Rabbit Polyclonal SMARCA1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: SMARCA1 Antibody [NB100-57524] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-57524 used for IP at 3 mcg/mg lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: SMARCA1 Antibody [NB100-57524] - Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody NB100-57524 (lot 2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMARCA1, NB100-57524 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: SMARCA1 Antibody [NB100-57524] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMARCA1/SNF2L used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB