22183-1-AP

antibody from Invitrogen Antibodies

Targeting: ARHGAP21 ARHGAP10, KIAA1424

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

22183-1-AP

Provider

Invitrogen Antibodies

Product name

ARHGAP21 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LDSSRLSPE VQSVAESKGD EADDERSELI SEGRPVETDS ESEFPVFPTA LTSERLFRGK LQEVTKSSRR NSEGSELSCT EGSLTSSLDS RRQLFSSHKL IECDTLSRKK SARFKSDSGS LGDAKNEKEA PSLTKVFDVM KKGKSTGSLL TPTRGESEKQ EPTWKTKIAD RLKLRPRAPA DDMFGVGNHK VNAETAKRKS IRRRHTLGGH RDATEISVLN FWKVHEQSGE RESELSAVNR LKPKCSAQDL SISDWLARER LRTSTSDLSR GEIGDPQTEN PSTREIATTD TPLSLHCNTG SSSSTLASTN RPLLSIPPQS PDQINGESFQ NVSKNASSAA NAQPHKLSET PGTKAEFHPC L (1598-1957 aa encoded by BC118915)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.55 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 22183-1-AP ( ARHGAP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 22183-1-AP ( ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).