PA5-143910

antibody from Invitrogen Antibodies

Targeting: SLC34A2 NAPI-3B

Western blot

Antibody data

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Validation data

Product number: PA5-143910 - Provider product page
Provider: Invitrogen Antibodies
Product name: SLC34A2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL. Positive Control - WB: human 293T whole cell, human HepG2 whole cell. IHC: human lung cancer tissue, mouse lung tissue, rat lung tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

SLC34A2 protein structure - PA5-143910 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SLC34A2 in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Samples were incubated with SLC34A2 Polyclonal antibody (Product # PA5-143910) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SLC34A2 in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Samples were incubated with SLC34A2 Polyclonal antibody (Product # PA5-143910) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SLC34A2 in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Samples were incubated with SLC34A2 Polyclonal antibody (Product # PA5-143910) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SLC34A2 in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Samples were incubated with SLC34A2 Polyclonal antibody (Product # PA5-143910) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.