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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- HPA043991 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA043991, RRID:AB_10966212
- Product name
- Anti-DBNDD2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DBNDD2, Gene description: dysbindin (dystrobrevin binding protein 1) domain containing 2, Alternative Gene Names: C20orf35, HSMNP1, Validated applications: ICC, IHC, WB, Uniprot ID: Q9BQY9, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes
Kelley K, Nakao-Inoue H, Molofsky A, Oldham M
Nature Neuroscience 2018;21(9):1171-1184
Nature Neuroscience 2018;21(9):1171-1184
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DBNDD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420786).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and kidney tissues using Anti-DBNDD2 antibody. Corresponding DBNDD2 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
