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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5618 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CLPX Antibody / Caseinolytic protease X
- Antibody type
- Monoclonal
- Description
- This highly specific CLPX antibody is suitable for use in Western blot/Immunofluorescence applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 11C11
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CLPX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Raji, 2) HeLa, 3) HepG2 and 4) Caco-2 lysate with CLPX antibody. Predicted molecular weight ~69 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Raji, 2) HeLa, 3) HepG2 and 4) Caco-2 lysate with CLPX antibody. Predicted molecular weight ~69 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human MCF7 cells with CLPX antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with CLPX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPX antibody.
