Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-36237 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLPX Monoclonal Antibody (11C11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human Raji tissue, human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell. ICC/IF: MCF7 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 11C11
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CLPX in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in CLPX monoclonal antibody (Product # MA5-36237) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CLPX in A549 cells. Samples were blocked with 10% normal goat serum and incubated in CLPX monoclonal antibody (Product # MA5-36237) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x106) and unlabeled sample (Red line).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CLPX in A549 cells. Samples were blocked with 10% normal goat serum and incubated in CLPX monoclonal antibody (Product # MA5-36237) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x106) and unlabeled sample (Red line).
