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- References [19]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 10794-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10794-1-AP, RRID:AB_2147391
- Product name
- MTHFD1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MTHFD1 antibody (Cat. #10794-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.
Discovery of 1,2,3,4-Tetrahydrochromeno[3,4-c]pyridin-5-one Derivatives as Novel MTHFD Inhibitors for the Treatment of Acute Myeloid Leukemia.
Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses.
Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging.
Evidence for interaction of 5,10-methylenetetrahydrofolate reductase (MTHFR) with methylenetetrahydrofolate dehydrogenase (MTHFD1) and general control nonderepressible 1 (GCN1).
Glutaminolysis is associated with mitochondrial pathway activation and can be therapeutically targeted in glioblastoma.
Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites.
MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma.
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
Newcastle Disease Virus Manipulates Mitochondrial MTHFD2-Mediated Nucleotide Metabolism for Virus Replication.
Cysteine is a limiting factor for glioma proliferation and survival.
Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
Illuminating NAD(+) Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.
The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2.
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Oxidative stress inhibits distant metastasis by human melanoma cells.
Kourtis S, Gañez Zapater A, Elbæk CR, Schmidt A, Ghose R, Coll Manzano A, Espinar Calvo L, Guirola M, Pardo-Lorente N, Garcia-Cao M, Pfeiffer M, Haynes S, Fontaine F, Müller A, Sdelci S
Nature communications 2026 Mar 6;17(1)
Nature communications 2026 Mar 6;17(1)
Discovery of 1,2,3,4-Tetrahydrochromeno[3,4-c]pyridin-5-one Derivatives as Novel MTHFD Inhibitors for the Treatment of Acute Myeloid Leukemia.
Lin H, He X, Sun B, Lu H, Liang J, Wang Q, Liao J, Yang X, Song Z, Zheng J, Lu W, Zhang A
Journal of medicinal chemistry 2026 Apr 9;69(7):8476-8502
Journal of medicinal chemistry 2026 Apr 9;69(7):8476-8502
Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses.
Meliton AY, Shin KWD, Cetin-Atalay R, Atalay MV, Tian Y, Houpy Szafran JC, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Dulin NO, Husain AN, Muir A, Shah H, Mutlu GM, Hamanaka RB
Nature communications 2025 Oct 20;16(1):9250
Nature communications 2025 Oct 20;16(1):9250
Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging.
Wu D, Tan B, Cheng Z, Li H, Li H, Yin Y, Ma F, Chen T, Dong X, Wang W, Hu Q
Nature communications 2025 Nov 3;16(1):9667
Nature communications 2025 Nov 3;16(1):9667
Evidence for interaction of 5,10-methylenetetrahydrofolate reductase (MTHFR) with methylenetetrahydrofolate dehydrogenase (MTHFD1) and general control nonderepressible 1 (GCN1).
Büchler LR, Blomgren LKM, Bürer C, Zanotelli VRT, Froese DS
Biochimie 2025 Mar;230:138-146
Biochimie 2025 Mar;230:138-146
Glutaminolysis is associated with mitochondrial pathway activation and can be therapeutically targeted in glioblastoma.
Miki K, Yagi M, Hatae R, Otsuji R, Miyazaki T, Goto K, Setoyama D, Fujioka Y, Sangatsuda Y, Kuga D, Higa N, Takajo T, Hajime Y, Akahane T, Tanimoto A, Hanaya R, Kunisaki Y, Uchiumi T, Yoshimoto K
Cancer & metabolism 2024 Nov 19;12(1):35
Cancer & metabolism 2024 Nov 19;12(1):35
Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites.
Yang L, Guo CW, Luo QM, Guo ZF, Chen L, Ishihama Y, Li P, Yang H, Gao W
Analytica chimica acta 2024 Jul 11;1312:342755
Analytica chimica acta 2024 Jul 11;1312:342755
MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma.
Guan J, Li M, Wang Y, Zhang Y, Que Y, Lu S, Wang J, Zhu J, Huang J, Zhen Z, Sun F, Song M, Zhang Y
Cell death & disease 2024 Feb 9;15(2):124
Cell death & disease 2024 Feb 9;15(2):124
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
Meliton AY, Cetin-Atalay R, Tian Y, Szafran JCH, Shin KWD, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Muir A, Mutlu GM, Hamanaka RB
bioRxiv : the preprint server for biology 2023 Nov 7;
bioRxiv : the preprint server for biology 2023 Nov 7;
Newcastle Disease Virus Manipulates Mitochondrial MTHFD2-Mediated Nucleotide Metabolism for Virus Replication.
Tang N, Chen P, Zhao C, Liu P, Tan L, Song C, Qiu X, Liao Y, Liu X, Luo T, Sun Y, Ding C
Journal of virology 2023 Mar 30;97(3):e0001623
Journal of virology 2023 Mar 30;97(3):e0001623
Cysteine is a limiting factor for glioma proliferation and survival.
Ruiz-Rodado V, Dowdy T, Lita A, Kramp T, Zhang M, Jung J, Dios-Esponera A, Zhang L, Herold-Mende CC, Camphausen K, Gilbert MR, Larion M
Molecular oncology 2022 May;16(9):1777-1794
Molecular oncology 2022 May;16(9):1777-1794
Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Anderson DE, Cui J, Ye Q, Huang B, Tan Y, Jiang C, Zu W, Gong J, Liu W, Kim SY, Yan BG, Sigmundsson K, Lim XF, Ye F, Niu P, Irving AT, Zhang H, Tang Y, Zhou X, Wang Y, Tan W, Wang LF, Tan X
Proceedings of the National Academy of Sciences of the United States of America 2021 Sep 28;118(39)
Proceedings of the National Academy of Sciences of the United States of America 2021 Sep 28;118(39)
Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.
Mitachi K, Ariake K, Shima H, Sato S, Miura T, Maeda S, Ishida M, Mizuma M, Ohtsuka H, Kamei T, Igarashi K, Unno M
Scientific reports 2021 Mar 22;11(1):6541
Scientific reports 2021 Mar 22;11(1):6541
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767
Journal of pineal research 2021 Dec;71(4):e12767
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
Shao C, Lu W, Du Y, Yan W, Bao Q, Tian Y, Wang G, Ye H, Hao H
Redox biology 2020 Sep;36:101685
Redox biology 2020 Sep;36:101685
Illuminating NAD(+) Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.
Zou Y, Wang A, Huang L, Zhu X, Hu Q, Zhang Y, Chen X, Li F, Wang Q, Wang H, Liu R, Zuo F, Li T, Yao J, Qian Y, Shi M, Yue X, Chen W, Zhang Z, Wang C, Zhou Y, Zhu L, Ju Z, Loscalzo J, Yang Y, Zhao Y
Developmental cell 2020 Apr 20;53(2):240-252.e7
Developmental cell 2020 Apr 20;53(2):240-252.e7
The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2.
Bao L, Festa F, Freet CS, Lee JP, Hirschler-Laszkiewicz IM, Chen SJ, Keefer KA, Wang HG, Patterson AD, Cheung JY, Miller BA
Scientific reports 2019 Oct 1;9(1):14132
Scientific reports 2019 Oct 1;9(1):14132
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Koufaris C, Nilsson R
Cancer & metabolism 2018;6:12
Cancer & metabolism 2018;6:12
Oxidative stress inhibits distant metastasis by human melanoma cells.
Piskounova E, Agathocleous M, Murphy MM, Hu Z, Huddlestun SE, Zhao Z, Leitch AM, Johnson TM, DeBerardinis RJ, Morrison SJ
Nature 2015 Nov 12;527(7577):186-91
Nature 2015 Nov 12;527(7577):186-91
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10794-1-AP(MTHFD1 antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MTHFD1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MTHFD1. This antibody recognizes human, mouse antigen. The MTHFD1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
