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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- HPA000704 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000704, RRID:AB_1079424
- Product name
- Anti-MTHFD1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human MTHFD1, Gene description: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase, Alternative Gene Names: MTHFC, MTHFD, Validated applications: IHC, WB, Uniprot ID: P11586, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells
CDK12 promotes tumorigenesis but induces vulnerability to therapies inhibiting folate one-carbon metabolism in breast cancer
Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency
Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs
Green A, Marttila P, Kiweler N, Chalkiadaki C, Wiita E, Cookson V, Lesur A, Eiden K, Bernardin F, Vallin K, Borhade S, Long M, Ghahe E, Jiménez-Alonso J, Jemth A, Loseva O, Mortusewicz O, Meyers M, Viry E, Johansson A, Hodek O, Homan E, Bonagas N, Ramos L, Sandberg L, Frödin M, Moussay E, Slipicevic A, Letellier E, Paggetti J, Sørensen C, Helleday T, Henriksson M, Meiser J
Nature Metabolism 2023;5(4):642-659
Nature Metabolism 2023;5(4):642-659
CDK12 promotes tumorigenesis but induces vulnerability to therapies inhibiting folate one-carbon metabolism in breast cancer
Filippone M, Gaglio D, Bonfanti R, Tucci F, Ceccacci E, Pennisi R, Bonanomi M, Jodice G, Tillhon M, Montani F, Bertalot G, Freddi S, Vecchi M, Taglialatela A, Romanenghi M, Romeo F, Bianco N, Munzone E, Sanguedolce F, Vago G, Viale G, Di Fiore P, Minucci S, Alberghina L, Colleoni M, Veronesi P, Tosoni D, Pece S
Nature Communications 2022;13(1)
Nature Communications 2022;13(1)
Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency
Zgheib R, Battaglia-Hsu S, Hergalant S, Quéré M, Alberto J, Chéry C, Rouyer P, Gauchotte G, Guéant J, Namour F
Cell Death & Disease 2019;10(8)
Cell Death & Disease 2019;10(8)
Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs
Tyleckova J, Hrabakova R, Mairychova K, Halada P, Radova L, Dzubak P, Hajduch M, Gadher S, Kovarova H
International Journal of Molecular Sciences 2012;13(12):15536-15564
International Journal of Molecular Sciences 2012;13(12):15536-15564
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
