PA5-102081

ADGRF3 antibody from Invitrogen Antibodies

GPR113, hGPCR37, PGR23

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for PA5-102081

Antibody Data

Product number

PA5-102081

Provider

Invitrogen Antibodies

Product name

GPR113 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Applications Tested: This Psel.KO2.3 antibody has been tested by flow cytometric analysis of thrombin-activated platelets. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GPR113 in LOVO cells. Samples were incubated with GPR113 polyclonal antibody (Product # PA5-102081).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GPR113 in LOVO cells. Samples were incubated with GPR113 polyclonal antibody (Product # PA5-102081).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPR113 in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GPR113 polyclonal antibody (Product # PA5-102081) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.