PA5-58249

antibody from Invitrogen Antibodies

Targeting: FAM13A ARHGAP48, FAM13A1, KIAA0914

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-58249

Provider

Invitrogen Antibodies

Product name

FAM13A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ESGTLSASSA TSARQRRRQS KEQDEVRHGR DKGLINKENT PSGFNHLDDC ILNTQEVEKV HKNTFGCAGE RS Highest antigen sequence identity to the following orthologs: Mouse - 54%, Rat - 47%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of FAM13A in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a FAM13A Polyclonal Antibody (Product # PA5-58249).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Protein FAM13A was performed using 70% confluent log phase 3T3-L1 cells differentiated to Adipocytes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FAM13A Polyclonal Antibody (Product # PA5-58249) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents undifferentiated 3T3L-1 cells showing lower expression of FAM13A. Panel f represents control 3T3L-1 cells with no primary antibody to assess background. The images were captured at 60X magnification.