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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-33036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFR alpha-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.
Peculis R, Mandrika I, Petrovska R, Dortane R, Megnis K, Nazarovs J, Balcere I, Stukens J, Konrade I, Pirags V, Klovins J, Rovite V
Frontiers in endocrinology 2020;11:313
Frontiers in endocrinology 2020;11:313
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GFR alpha-2 was performed by loading HeLa cell lysate. Proteins were transferred to a membrane and probed with a GFR alpha-2 Polyclonal Antibody (Product # PA1-33036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GFR alpha-2 in Hela cells using a GFR alpha-2 polyclonal antibody (Product # PA1-33036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Expression of cell markers in pituispheres. Representative immunofluorescence images of (A) PA02, (B) PA03, and (C) PA05 pituispheres. Cells were double-stained for (A,B) CD15 (green) and GFRalpha2 (red), (C) Pit-1 (green), and SF-1 (red) (B) CD15 (green) and SF-1 (red) and PA02 pituispheres were single stained for (A) Pit-1 (green) and SF-1 (red) and PA05 pituispheres were single stained for (C) Pit-1 (green) and GFRalpha2 (red). Isotype-matched antibodies were used for negative controls. Cell nuclei were counterstained with DAPI (blue). Scale bar, 13 mum.
