PA5-104160

antibody from Invitrogen Antibodies

Targeting: SLC9C1 NHE, SLC9A10

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-104160

Provider

Invitrogen Antibodies

Product name

SLC9A10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total SLC9A10.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

The sodium/proton exchanger SLC9C1 (sNHE) is essential for human sperm motility and fertility.

Cavarocchi E, Whitfield M, Chargui A, Stouvenel L, Lorès P, Coutton C, Arnoult C, Santulli P, Patrat C, Thierry-Mieg N, Ray PF, Dulioust E, Touré A
Clinical genetics 2021 May;99(5):684-693

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 FIGURE Analysis of SLC9C1 protein in sperm from the patient carrying the c.2748 + 2 T > C mutation. (A) SLC9C1 protein detection (red) by immunofluorescence assay in sperm cells from control individual (top) and patient (bottom). Two different antibodies were used: one antibody directed against the central region of SLC9C1 and a second antibody directed against the C-terminal region and including exon 22 coding region. Co-staining with alpha-tubulin antibody (green) was also performed. The protein localises at the midpiece and principal piece of sperm flagella, with a decreasing gradient towards the end of the tail. The central antibody allowed to confirm presence of SLC9C1 in patient spermatozoa, while absence of signal of the C-terminal antibody confirmed an abnormal protein lacking part of the CNBD. Scale bars represent 10 mum. (B) Immunoblotting detection of SLC9C1 protein (135 kDa) in sperm lysate from control individual (left) and patient (right). beta-tubulin (55 kDa) was used for normalization