PA5-58351

antibody from Invitrogen Antibodies

Targeting: BUD13 Cwc26, fSAP71, MGC13125

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58351

Provider

Invitrogen Antibodies

Product name

BUD13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PGHQDSDSDL SPPRNRPRHR SSDSDLSPPR RRQRTKSSDS DLSPPRRSQP PGKKAAHMYS GAKTGLVLTD IQREQQELKE QDQETM Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 81%.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

CircSERPINA3 regulates SERPINA3-mediated apoptosis, autophagy and aerobic glycolysis of prostate cancer cells by competitively binding to MiR-653-5p and recruiting BUD13.

Xing Z, Li S, Liu Z, Zhang C, Bai Z
Journal of translational medicine 2021 Dec 3;19(1):492

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of BUD13 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a BUD13 Polyclonal Antibody (Product # PA5-58351).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BUD13 in human kidney using BUD13 Polyclonal Antibody (Product # PA5-58351).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BUD13 in human cerebral cortex, colon, kidney and testis using BUD13 Polyclonal Antibody (Product # PA5-58351) (A) shows similar protein distribution across tissues to an independent BUD13 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BUD13 in human testis using BUD13 Polyclonal Antibody (Product # PA5-58351).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BUD13 in human colon using BUD13 Polyclonal Antibody (Product # PA5-58351).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BUD13 in human cerebral cortex using BUD13 Polyclonal Antibody (Product # PA5-58351).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 CircSERPINA3 stabilizes SERPINA3 via recruiting BUD13. A RIP assay was used to examine the enrichment of circSERPINA3 and SERPINA3 in PC-3 cells incubated with different antibodies of candidate shared RBP screened out from starBase. B , C Western blot analysis was conducted to examine BUD13 expression when circSERPINA3 was down-regulated or overexpressed in PC-3 cells. D , E RT-qPCR was used to examine the knockdown efficiency of sh-BUD13-1/2/3 and overexpression efficiency of pcDNA3.1-BUD13 in PCa cells. F , G RT-qPCR was carried out to examine SERPINA3 expression when BUD13 was reduced or overexpressed in PC-3 cells. H , I RT-qPCR was used to examine the effect of sh-BUD13-1 on half-life of SERPINA3 and beta-actin in PC-3 cells. J , K Expression of SERPINA3 and beta-actin in PC-3 cells was tested by means of RT-qPCR after cells were transfected with pcDNA3.1-BUD13. ( sh short hairpin, NC negative control). ** P < 0.01