TA503291

antibody from OriGene

Targeting: DPP3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503291

Provider

OriGene

Proper citation

OriGene Cat#TA503291, RRID:AB_11123918

Product name

DPP3 mouse monoclonal antibody, clone OTI 2G8 (formerly 2G8)

Antibody type

Monoclonal

Description

DPP3 mouse monoclonal antibody, clone OTI 2G8 (formerly 2G8)

Host

Mouse

Conjugate

Unconjugated

Epitope

DPP3

Isotype

IgG

Antibody clone number

OTI2G8

Vial size

100 µl

Concentration

0.13 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP3 (RC219658, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP3.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC219658 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DPP3 antibody(TA503291), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-DPP3 antibody(TA503291),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC