Explore
Validate
LearnGTX24194
antibody from GeneTex
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX24194 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24194, RRID:AB_370110
- Product name
- DNA-PKcs (phospho Thr2609) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Nuclear localized Raf1 isoform alters DNA-dependent protein kinase activity and the DNA damage response.
Kinomic profiling approach identifies Trk as a novel radiation modulator.
MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma.
Cetuximab augments cytotoxicity with poly (adp-ribose) polymerase inhibition in head and neck cancer.
Nixon BR, Sebag SC, Glennon MS, Hall EJ, Kounlavong ES, Freeman ML, Becker JR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jan;33(1):1138-1150
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jan;33(1):1138-1150
Kinomic profiling approach identifies Trk as a novel radiation modulator.
Jarboe JS, Jaboin JJ, Anderson JC, Nowsheen S, Stanley JA, Naji F, Ruijtenbeek R, Tu T, Hallahan DE, Yang ES, Bonner JA, Willey CD
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2012 Jun;103(3):380-7
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2012 Jun;103(3):380-7
MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma.
Jarboe JS, Anderson JC, Duarte CW, Mehta T, Nowsheen S, Hicks PH, Whitley AC, Rohrbach TD, McCubrey RO, Chiu S, Burleson TM, Bonner JA, Gillespie GY, Yang ES, Willey CD
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jun 1;18(11):3030-41
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jun 1;18(11):3030-41
Cetuximab augments cytotoxicity with poly (adp-ribose) polymerase inhibition in head and neck cancer.
Nowsheen S, Bonner JA, Lobuglio AF, Trummell H, Whitley AC, Dobelbower MC, Yang ES
PloS one 2011;6(8):e24148
PloS one 2011;6(8):e24148
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody (GTX24194) shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ?g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
