STJ99135
antibody from St John's Laboratory
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- STJ99135 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PRKDC antibody [8D3-B12-F11] (STJ99135)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Dna-Dependent Protein Kinase Catalytic Subunit is suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry research applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of DNA-PKcs in K562, Molt-4, A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of DNA-PKcs in Hela, K562, HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa, Observed band size:450KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:200).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA