A303-967A
antibody from Invitrogen Antibodies
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- A303-967A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNA-PKcs Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DNA-PKcs by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat anti-DNA-PKcs antibody A303-967A (lot A303-967A-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated DNA-PKcs, A303-967A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DNA-PKcs by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified goat anti-DNA-PKcs antibody A303-967A (lot A303-967A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DNA-PKcs by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- DNA-PKcs (Cat. No. A303-967A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.