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LearnMA5-13235
antibody from Invitrogen Antibodies
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [49]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-13235 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNA-PK Monoclonal Antibody (18-2), Biotin
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-13235 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-13235 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 18-2
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references p21 promotes error-free replication-coupled DNA double-strand break repair.
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Gene amplification in human cells knocked down for RAD54.
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
Defective Artemis causes mild telomere dysfunction.
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
ARTEMIS nuclease facilitates apoptotic chromatin cleavage.
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis.
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Long-term XPC silencing reduces DNA double-strand break repair.
The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.
DNA end joining activity is reduced in Alzheimer's disease.
Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.
Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Backup pathways of NHEJ are suppressed by DNA-PK.
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Mauro M, Rego MA, Boisvert RA, Esashi F, Cavallo F, Jasin M, Howlett NG
Nucleic acids research 2012 Sep 1;40(17):8348-60
Nucleic acids research 2012 Sep 1;40(17):8348-60
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
Kang KB, Zhu C, Wong YL, Gao Q, Ty A, Wong MC
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, de Stefano MC, Pocchiari M, Garaci E, Merlo D
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
Gene amplification in human cells knocked down for RAD54.
Ruiz-Herrera A, Smirnova A, Khoriauli L, Nergadze SG, Mondello C, Giulotto E
Genome integrity 2011 Mar 18;2(1):5
Genome integrity 2011 Mar 18;2(1):5
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, Salles B, Muller C
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Yu X, Wang H, Wang P, Chen BP, Wang Y
Journal of cellular physiology 2011 Feb;226(2):369-74
Journal of cellular physiology 2011 Feb;226(2):369-74
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, Ren GS, Salles B, Calsou P
Nucleic acids research 2011 Dec;39(22):9605-19
Nucleic acids research 2011 Dec;39(22):9605-19
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
Takai H, Xie Y, de Lange T, Pavletich NP
Genes & development 2010 Sep 15;24(18):2019-30
Genes & development 2010 Sep 15;24(18):2019-30
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
Papeta N, Zheng Z, Schon EA, Brosel S, Altintas MM, Nasr SH, Reiser J, D'Agati VD, Gharavi AG
The Journal of clinical investigation 2010 Nov;120(11):4055-64
The Journal of clinical investigation 2010 Nov;120(11):4055-64
Defective Artemis causes mild telomere dysfunction.
Yasaei H, Slijepcevic P
Genome integrity 2010 May 26;1(1):3
Genome integrity 2010 May 26;1(1):3
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Choi YK, Nash K, Byrne BJ, Muzyczka N, Song S
PloS one 2010 Dec 20;5(12):e15073
PloS one 2010 Dec 20;5(12):e15073
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Velkova A, Carvalho MA, Johnson JO, Tavtigian SV, Monteiro AN
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
ARTEMIS nuclease facilitates apoptotic chromatin cleavage.
Britton S, Frit P, Biard D, Salles B, Calsou P
Cancer research 2009 Oct 15;69(20):8120-6
Cancer research 2009 Oct 15;69(20):8120-6
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
Daya S, Cortez N, Berns KI
Journal of virology 2009 Nov;83(22):11655-64
Journal of virology 2009 Nov;83(22):11655-64
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
Choul-li S, Drobecq H, Aumercier M
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis.
Meulle A, Salles B, Daviaud D, Valet P, Muller C
PloS one 2008;3(10):e3345
PloS one 2008;3(10):e3345
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
Schild-Poulter C, Su A, Shih A, Kelly OP, Fritzler MJ, Goldstein R, Haché RJ
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Hégarat N, Cardoso GM, Rusconi F, Francois JC, Praseuth D
Nucleic acids research 2007;35(13):e92
Nucleic acids research 2007;35(13):e92
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Wu PY, Frit P, Malivert L, Revy P, Biard D, Salles B, Calsou P
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
Long-term XPC silencing reduces DNA double-strand break repair.
Despras E, Pfeiffer P, Salles B, Calsou P, Kuhfittig-Kulle S, Angulo JF, Biard DS
Cancer research 2007 Mar 15;67(6):2526-34
Cancer research 2007 Mar 15;67(6):2526-34
The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.
Kiefer K, Oshinsky J, Kim J, Nakajima PB, Bosma GC, Bosma MJ
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 20;104(8):2843-8
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 20;104(8):2843-8
DNA end joining activity is reduced in Alzheimer's disease.
Shackelford DA
Neurobiology of aging 2006 Apr;27(4):596-605
Neurobiology of aging 2006 Apr;27(4):596-605
Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.
Repasky JA, Corbett E, Boboila C, Schatz DG
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5478-88
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5478-88
Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.
Repasky JA, Corbett E, Boboila C, Schatz DG
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5478-88
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5478-88
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Loong SL, Korzh S, Price A
Oncogene 2004 Jul 15;23(32):5562-6
Oncogene 2004 Jul 15;23(32):5562-6
Backup pathways of NHEJ are suppressed by DNA-PK.
Perrault R, Wang H, Wang M, Rosidi B, Iliakis G
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay JP
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Song S, Lu Y, Choi YK, Han Y, Tang Q, Zhao G, Berns KI, Flotte TR
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L
Experimental and molecular pathology 2004 Aug;77(1):1-6
Experimental and molecular pathology 2004 Aug;77(1):1-6
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Wang H, Perrault AR, Takeda Y, Qin W, Wang H, Iliakis G
Nucleic acids research 2003 Sep 15;31(18):5377-88
Nucleic acids research 2003 Sep 15;31(18):5377-88
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Wang H, Perrault AR, Takeda Y, Qin W, Wang H, Iliakis G
Nucleic acids research 2003 Sep 15;31(18):5377-88
Nucleic acids research 2003 Sep 15;31(18):5377-88
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Udayakumar D, Bladen CL, Hudson FZ, Dynan WS
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Guéret M
Nucleic acids research 2003 Nov 1;31(21):6272-82
Nucleic acids research 2003 Nov 1;31(21):6272-82
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Guéret M
Nucleic acids research 2003 Nov 1;31(21):6272-82
Nucleic acids research 2003 Nov 1;31(21):6272-82
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Lebrasseur NK, Coté GM, Miller TA, Fielding RA, Sawyer DB
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Friesland S, Kanter-Lewensohn L, Tell R, Munck-Wikland E, Lewensohn R, Nilsson A
Head & neck 2003 Apr;25(4):313-21
Head & neck 2003 Apr;25(4):313-21
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Cho NH, Cordon-Cardo C, Li GC, Kim SH
The Journal of pathology 2002 Sep;198(1):21-9
The Journal of pathology 2002 Sep;198(1):21-9
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ma Y, Pannicke U, Schwarz K, Lieber MR
Cell 2002 Mar 22;108(6):781-94
Cell 2002 Mar 22;108(6):781-94
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ma Y, Pannicke U, Schwarz K, Lieber MR
Cell 2002 Mar 22;108(6):781-94
Cell 2002 Mar 22;108(6):781-94
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
Brown KD, Lataxes TA, Shangary S, Mannino JL, Giardina JF, Chen J, Baskaran R
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Jhappan C, Yusufzai TM, Anderson S, Anver MR, Merlino G
Molecular and cellular biology 2000 Jun;20(11):4075-83
Molecular and cellular biology 2000 Jun;20(11):4075-83
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Jhappan C, Yusufzai TM, Anderson S, Anver MR, Merlino G
Molecular and cellular biology 2000 Jun;20(11):4075-83
Molecular and cellular biology 2000 Jun;20(11):4075-83
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Chen L, Trujillo K, Sung P, Tomkinson AE
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y
The EMBO journal 1999 Mar 1;18(5):1397-406
The EMBO journal 1999 Mar 1;18(5):1397-406
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Giffin W, Gong W, Schild-Poulter C, Haché RJ
Molecular and cellular biology 1999 Jun;19(6):4065-78
Molecular and cellular biology 1999 Jun;19(6):4065-78
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Giffin W, Gong W, Schild-Poulter C, Haché RJ
Molecular and cellular biology 1999 Jun;19(6):4065-78
Molecular and cellular biology 1999 Jun;19(6):4065-78
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver DT, Alt FW
Immunity 1998 Sep;9(3):367-76
Immunity 1998 Sep;9(3):367-76
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver DT, Alt FW
Immunity 1998 Sep;9(3):367-76
Immunity 1998 Sep;9(3):367-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13235) on LS174T Cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with DNAKcs antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13235) on denatured Human LS174T Cells.
- Conjugate
- Biotin
