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LearnMA5-13244
antibody from Invitrogen Antibodies
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blot
Gel shiftAntibody data
- Antibody Data
- Antigen structure
- References [46]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
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- Product number
- MA5-13244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNA-PK Monoclonal Antibody (25-4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-13244 targets DNA-PKcs in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with Human samples. The MA5-13244 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 25-4
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references p21 promotes error-free replication-coupled DNA double-strand break repair.
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
Intranuclear delivery of a novel antibody-derived radiosensitizer targeting the DNA-dependent protein kinase catalytic subunit.
Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection.
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Gene amplification in human cells knocked down for RAD54.
Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia.
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor.
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends.
Defective Artemis causes mild telomere dysfunction.
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
DNA-dependent protein kinase is a therapeutic target and an indicator of poor prognosis in B-cell chronic lymphocytic leukemia.
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells.
Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection.
The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM.
DNA end joining activity is reduced in Alzheimer's disease.
Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase.
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Backup pathways of NHEJ are suppressed by DNA-PK.
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites.
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Mauro M, Rego MA, Boisvert RA, Esashi F, Cavallo F, Jasin M, Howlett NG
Nucleic acids research 2012 Sep 1;40(17):8348-60
Nucleic acids research 2012 Sep 1;40(17):8348-60
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
Kang KB, Zhu C, Wong YL, Gao Q, Ty A, Wong MC
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
Intranuclear delivery of a novel antibody-derived radiosensitizer targeting the DNA-dependent protein kinase catalytic subunit.
Xiong H, Lee RJ, Haura EB, Edwards JG, Dynan WS, Li S
International journal of radiation oncology, biology, physics 2012 Jul 1;83(3):1023-30
International journal of radiation oncology, biology, physics 2012 Jul 1;83(3):1023-30
Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection.
Vogel R, Seyffert M, Strasser R, de Oliveira AP, Dresch C, Glauser DL, Jolinon N, Salvetti A, Weitzman MD, Ackermann M, Fraefel C
Journal of virology 2012 Jan;86(1):143-55
Journal of virology 2012 Jan;86(1):143-55
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, de Stefano MC, Pocchiari M, Garaci E, Merlo D
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
Gene amplification in human cells knocked down for RAD54.
Ruiz-Herrera A, Smirnova A, Khoriauli L, Nergadze SG, Mondello C, Giulotto E
Genome integrity 2011 Mar 18;2(1):5
Genome integrity 2011 Mar 18;2(1):5
Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia.
Elliott SL, Crawford C, Mulligan E, Summerfield G, Newton P, Wallis J, Mainou-Fowler T, Evans P, Bedwell C, Durkacz BW, Willmore E
British journal of haematology 2011 Jan;152(1):61-71
British journal of haematology 2011 Jan;152(1):61-71
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Yu X, Wang H, Wang P, Chen BP, Wang Y
Journal of cellular physiology 2011 Feb;226(2):369-74
Journal of cellular physiology 2011 Feb;226(2):369-74
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
Takai H, Xie Y, de Lange T, Pavletich NP
Genes & development 2010 Sep 15;24(18):2019-30
Genes & development 2010 Sep 15;24(18):2019-30
MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor.
Johns TG, McKay MJ, Cvrljevic AN, Gan HK, Taylor C, Xu H, Smyth FE, Scott AM
International journal of radiation oncology, biology, physics 2010 Oct 1;78(2):572-8
International journal of radiation oncology, biology, physics 2010 Oct 1;78(2):572-8
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
Papeta N, Zheng Z, Schon EA, Brosel S, Altintas MM, Nasr SH, Reiser J, D'Agati VD, Gharavi AG
The Journal of clinical investigation 2010 Nov;120(11):4055-64
The Journal of clinical investigation 2010 Nov;120(11):4055-64
TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends.
Bombarde O, Boby C, Gomez D, Frit P, Giraud-Panis MJ, Gilson E, Salles B, Calsou P
The EMBO journal 2010 May 5;29(9):1573-84
The EMBO journal 2010 May 5;29(9):1573-84
Defective Artemis causes mild telomere dysfunction.
Yasaei H, Slijepcevic P
Genome integrity 2010 May 26;1(1):3
Genome integrity 2010 May 26;1(1):3
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Choi YK, Nash K, Byrne BJ, Muzyczka N, Song S
PloS one 2010 Dec 20;5(12):e15073
PloS one 2010 Dec 20;5(12):e15073
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Velkova A, Carvalho MA, Johnson JO, Tavtigian SV, Monteiro AN
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Velkova A, Carvalho MA, Johnson JO, Tavtigian SV, Monteiro AN
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
Daya S, Cortez N, Berns KI
Journal of virology 2009 Nov;83(22):11655-64
Journal of virology 2009 Nov;83(22):11655-64
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
Choul-li S, Drobecq H, Aumercier M
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
DNA-dependent protein kinase is a therapeutic target and an indicator of poor prognosis in B-cell chronic lymphocytic leukemia.
Willmore E, Elliott SL, Mainou-Fowler T, Summerfield GP, Jackson GH, O'Neill F, Lowe C, Carter A, Harris R, Pettitt AR, Cano-Soumillac C, Griffin RJ, Cowell IG, Austin CA, Durkacz BW
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jun 15;14(12):3984-92
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jun 15;14(12):3984-92
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
Schild-Poulter C, Su A, Shih A, Kelly OP, Fritzler MJ, Goldstein R, Haché RJ
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells.
Zhong X, Safa AR
Biochemistry 2007 May 15;46(19):5766-75
Biochemistry 2007 May 15;46(19):5766-75
Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection.
Luo MH, Rosenke K, Czornak K, Fortunato EA
Journal of virology 2007 Feb;81(4):1934-50
Journal of virology 2007 Feb;81(4):1934-50
The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM.
Jiang X, Sun Y, Chen S, Roy K, Price BD
The Journal of biological chemistry 2006 Jun 9;281(23):15741-6
The Journal of biological chemistry 2006 Jun 9;281(23):15741-6
DNA end joining activity is reduced in Alzheimer's disease.
Shackelford DA
Neurobiology of aging 2006 Apr;27(4):596-605
Neurobiology of aging 2006 Apr;27(4):596-605
Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase.
Lau A, Swinbank KM, Ahmed PS, Taylor DL, Jackson SP, Smith GC, O'Connor MJ
Nature cell biology 2005 May;7(5):493-500
Nature cell biology 2005 May;7(5):493-500
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Loong SL, Korzh S, Price A
Oncogene 2004 Jul 15;23(32):5562-6
Oncogene 2004 Jul 15;23(32):5562-6
Backup pathways of NHEJ are suppressed by DNA-PK.
Perrault R, Wang H, Wang M, Rosidi B, Iliakis G
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay JP
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Song S, Lu Y, Choi YK, Han Y, Tang Q, Zhao G, Berns KI, Flotte TR
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L
Experimental and molecular pathology 2004 Aug;77(1):1-6
Experimental and molecular pathology 2004 Aug;77(1):1-6
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Wang H, Perrault AR, Takeda Y, Qin W, Wang H, Iliakis G
Nucleic acids research 2003 Sep 15;31(18):5377-88
Nucleic acids research 2003 Sep 15;31(18):5377-88
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Udayakumar D, Bladen CL, Hudson FZ, Dynan WS
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Guéret M
Nucleic acids research 2003 Nov 1;31(21):6272-82
Nucleic acids research 2003 Nov 1;31(21):6272-82
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Lebrasseur NK, Coté GM, Miller TA, Fielding RA, Sawyer DB
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Calsou P, Delteil C, Frit P, Drouet J, Salles B
Journal of molecular biology 2003 Feb 7;326(1):93-103
Journal of molecular biology 2003 Feb 7;326(1):93-103
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Friesland S, Kanter-Lewensohn L, Tell R, Munck-Wikland E, Lewensohn R, Nilsson A
Head & neck 2003 Apr;25(4):313-21
Head & neck 2003 Apr;25(4):313-21
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Cho NH, Cordon-Cardo C, Li GC, Kim SH
The Journal of pathology 2002 Sep;198(1):21-9
The Journal of pathology 2002 Sep;198(1):21-9
Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites.
Mo X, Dynan WS
Molecular and cellular biology 2002 Nov;22(22):8088-99
Molecular and cellular biology 2002 Nov;22(22):8088-99
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ma Y, Pannicke U, Schwarz K, Lieber MR
Cell 2002 Mar 22;108(6):781-94
Cell 2002 Mar 22;108(6):781-94
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
Brown KD, Lataxes TA, Shangary S, Mannino JL, Giardina JF, Chen J, Baskaran R
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Jhappan C, Yusufzai TM, Anderson S, Anver MR, Merlino G
Molecular and cellular biology 2000 Jun;20(11):4075-83
Molecular and cellular biology 2000 Jun;20(11):4075-83
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Chen L, Trujillo K, Sung P, Tomkinson AE
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y
The EMBO journal 1999 Mar 1;18(5):1397-406
The EMBO journal 1999 Mar 1;18(5):1397-406
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Giffin W, Gong W, Schild-Poulter C, Haché RJ
Molecular and cellular biology 1999 Jun;19(6):4065-78
Molecular and cellular biology 1999 Jun;19(6):4065-78
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver DT, Alt FW
Immunity 1998 Sep;9(3):367-76
Immunity 1998 Sep;9(3):367-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13244) on LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA-PKcs was performed by loading 30 µg of MiaPaCa-2 lysate (lane 1), 30 µg of OVCAR-8 lysate (lane 2) and 30 µg of MDA-MB-231 lysate (lane 3) in reducing sample buffer and Page Rµler Protein Ladder (Product # 26616) onto a 6% polyacrylamide gel. Proteins were transferred to PVDF membrane using the wet transfer method. Membrane was blocked in Super Block Blocking Buffer (Product # 37515). DNA-PKcs was detected at approximately 469 kDa using a DNA-PKcs monoclonal antibody (Product # MA5-13244) at a dilution of 1:1000 in 5% BSA, followed by a goat anti-mouse IgG DyLight 800 conjugated secondary antibody (Product # SA5-35521) at a dilution of 1:7500. Data courtesy of Antibody Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (MA5-13244) on denatured Human LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (MA5-13244) on Native Human LS174T Cells.
