Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-21071 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC39A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse lung tissue lysate. PA5-21071 can be used with blocking peptide PEP-1185.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.
Prolonged stimulation of insulin release from MIN6 cells causes zinc depletion and loss of β-cell markers.
Protic I, Golic I, Vidakovic S, Korac B, Korac A
Current issues in molecular biology 2022 Jul 31;44(8):3444-3454
Current issues in molecular biology 2022 Jul 31;44(8):3444-3454
Prolonged stimulation of insulin release from MIN6 cells causes zinc depletion and loss of β-cell markers.
Lawson R, Maret W, Hogstrand C
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2018 Sep;49:51-59
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2018 Sep;49:51-59
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse lung tissue lysate using a ZIP6 polyclonal antibody (Product # PA5-21071) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZIP6 in mouse lung tissue lysate with SLC39A6 Polyclonal Antibody (Product # PA5-21071) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZIP6 in mouse lung tissue with SLC39A6 Polyclonal Antibody (Product # PA5-21071) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ZIP6 in mouse lung tissue with SLC39A6 Polyclonal Antibody (Product # PA5-21071) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence detection of ZIP6 transporter in normozoospermic ( a ) and asthenozoospermic ( c ) sperm samples using a confocal laser scanning microscope. Sub-compartments of anterior head region: acorn-like ( 1 ), crescent-like ( 2 ), acorn/crescent-like ( 3 ) patterns and posterior head region. Nc--negative control. Scale bar = 10 um. Average of total number of spermatozoa per ejaculate before processing was in normo- (625 x 10 6 /vol) and asthenozoospermia (367.5 x 10 6 /vol); For each type of sample, six randomly selected field areas were analysed (>100 cells). Relative immunopositivity of ZIP6 among the compartments in the normozoospermic ( b ) and asthenozoospermic ( d ) sperm samples. * in comparison to head, * p