HPA064843

antibody from Atlas Antibodies

Targeting: SPICE1 CCDC52, SPICE

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA064843

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA064843, RRID:AB_2685367

Product name

Anti-SPICE1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SPICE1, Gene description: spindle and centriole associated protein 1, Alternative Gene Names: CCDC52, SPICE, Validated applications: WB, IHC, ICC, Uniprot ID: Q8N0Z3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.05 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.

Balestra FR, Domínguez-Calvo A, Wolf B, Busso C, Buff A, Averink T, Lipsanen-Nyman M, Huertas P, Ríos RM, Gönczy P
eLife 2021 Jan 25;10

---

A rapid computational approach identifies SPICE1 as an Aurora kinase substrate.

Deretic J, Kerr A, Welburn JPI
Molecular biology of the cell 2019 Feb 1;30(3):312-323

---

PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN

Sydor A, Coyaud E, Rovelli C, Laurent E, Liu H, Raught B, Mennella V
eLife 2018;7

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SPICE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408168).

Sample type

Human

Protocol

Protocol