PAB20167

antibody from Abnova Corporation

Targeting: EBP CDPX2, CHO2, CPX, CPXD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20167

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20167, RRID:AB_10962011

Product name

EBP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant EBP.

Antigen sequence

YEDLLGDQAFLSQLWKEYAKGDSRYILGDNF


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with EBP polyclonal antibody (Cat # PAB20167) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with EBP polyclonal antibody (Cat # PAB20167) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with EBP polyclonal antibody (Cat # PAB20167) shows strong cytoplasmic positivity in Leydig cells.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)