PA5-57623

antibody from Invitrogen Antibodies

Targeting: ARFGEF3 BIG3, C6orf92, dJ171N11.1, KIAA1244, PPP1R33

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57623

Provider

Invitrogen Antibodies

Product name

ARFGEF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SLKLLKNQEA DQHSARLFIQ SLEGLLPRLL SLSNVEEVDT ALQNFASTFC SGMMHSPGFD GNSSLSFQML MNADSLYTAA HCALLLNLKL SHG Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 95%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.09 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation.

Gillingham AK, Bertram J, Begum F, Munro S
eLife 2019 Jul 11;8

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7. Validation of putative effectors for Rab2A, Rab5A and Rab9A. ( A ) Affinity chromatography of cell lysates (input) using GST-fusions to the indicated GTP-locked (QL) or GDP-locked Rabs. The bound proteins were eluted and probed with antibodies to the indicated proteins. Lysates were from HEK293 cells and probed for STAMBPL1, or from rat brain and probed for ARFGEF3. ( B ) Confocal micrographs of MEFs expressing either GFP-STAMPBL1 alone and probed for a Golgi marker, or co-expressing the mitochondrial Rab2A(Q65L)-BirA* as indicated. ( C ) As ( B ) except that the cells were expressing GFP-ARFGEF3. ( D ) Coomassie blue stained gel showing affinity chromatography of purified recombinant GFP-STAMBPL1 using GST fusions to the indicate GTP-locked (QL) or GDP-locked (SN) Rabs. The recombinant GFP-STAMBPL1 binds specifically to the GTP form of Rab2A. ( E ) Affinity chromatography of HeLa cell lysates (input) using GST-fusions to the indicated GTP-locked (QL) or GDP-locked Rabs. The bound proteins were eluted and probed with antibodies to the indicated proteins. TBCK, OSBPL9 and RELCH show specific binding to the GTP form of Rab5, with RELCH also binding to Rab11-GTP as previously reported ( Sobajima et al., 2018 ). ( F ) Confocal micrographs of cells expressing GFP-TBCK alone, or with co-expression of mitochondrial Rab5A(Q79L)-BirA* as indicated. ( G ) Coomassie blue stained gel showing affinity chromatography of lysate from E. coli expressing His 6 -Nde1 (input) using