Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NXF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Chimpanzee (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), IMR32 (Lane 2), Hep G2 (Lane 3) and U-2 OS (Lane 4). The blot was probed with Anti-NXF3 Polyclonal Antibody (Product # PA5-30700, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 60 kDa band corresponding to NXF3 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NXF3 Polyclonal Antibody (Product # PA5-30700). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: IMR32. Lane C: U87-MG. Lane D: MCF-7 . 7.5% SDS PAGE. NXF3 Polyclonal Antibody (Product # PA5-30700) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NXF3 was achieved by transfecting U-87 MG cells with NXF3 specific siRNAs (Silencer® select Product # s31847, s224404). Western blot analysis (Fig. a) was performed using whole cell extracts from the NXF3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with NXF3 Polyclonal Antibody (Product # PA5-30700, 1:4000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NXF3.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NXF3 in paraformaldehyde-fixed MCF-7 cells using a NXF3 polyclonal antibody (Product # PA5-30700) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3 (Product # PA5-30700) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.