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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-87606 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIR3DL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QDKPFLSAWP GTVVSEGQHV TLQCRSRLGF NEFSLSKEDG MPVPELYNRI FRNSFLMGPV TPAHAGTYRC CSSHPHSPTG WSAPSNPVVI MVTGVHRKPS LLAHPGPLVK SGETVILQCW SDVRFERFLL HREGITEDPL RLVGQLHDAG SQVNYSMGPM TPALAGTYRC FGSVTHLPYE LSAPSDPLDI VVVGLYGKPS LSAQPGPTVQ AGENVTLSCS SRSLFDIYHL SREAEAGELR LTAVLRVNGT FQANFPLGPV THGGNYRCFG SFRALPHAWS DPSDPLPVSV TGNSRNL; Positive Samples: HepG2, SGC-7901; Cellular Location: Cell membrane, Single-pass type I membrane protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.14 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KIR3DL3 in extracts of various cell lines. Samples were incubated with KIR3DL3 Polyclonal antibody (Product # PA5-87606) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIR3DL3 in paraffin-embedded human colon carcinoma. Samples were incubated with KIR3DL3 Polyclonal antibody (Product # PA5-87606) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIR3DL3 in paraffin-embedded human appendix. Samples were incubated with KIR3DL3 Polyclonal antibody (Product # PA5-87606) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIR3DL3 in paraffin-embedded human appendix. Samples were incubated with KIR3DL3 Polyclonal antibody (Product # PA5-87606) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
